In-Silico Structural and Functional Characterization of WsMYB44 Protein from Withania somnifera L. Dunal

The plant based MYB transcription factors have been extensively studied for structural and functional characterization. protein is known to perform several molecular biological processes including regulation of gene expression. In the current study, we identified characterized a R2R3 type TF (WsMYB44) pertaining root specific library from Withania somnifera. phylogenetic studies sequence revealed its recent origin Nicotiana sp., member Solanaceae family. Motif scans domain analysis depicted strong conservation near signature R2 R3 DNA binding domains which proved specificity WsMYB44 protein. modeled 3-D structure showed significant affinity with element C/TAACG/TG. synergistic act predicted was by studying protein-protein interaction networks. Furthermore, Gene Ontology terms nuclear localization found be involved in defense related signaling cascades.